Premium NAD+ 500MG Research Coenzyme — High-Purity Nicotinamide Adenine Dinucleotide for Laboratory Use

Product Overview

Premium NAD+ (nicotinamide adenine dinucleotide) 500MG is a research‑grade coenzyme essential for cellular metabolism and energy production. It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 500mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 500mg per vial, 10 vials per pack

• Form: Lyophilized (freeze‑dried) powder

• Purity: ≥99% verified by third‑party testing

• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂

• Molecular Mass: 663.43

• CAS Number: 53-84-9

• Synonyms: DPN, Cozymase, Codehydrogenase I, Nadide, Beta‑NAD

• Research Use Only: Not for human or animal consumption

Understanding NAD+: The Research Context

NAD+ (nicotinamide adenine dinucleotide) is a fundamental coenzyme found in every living cell. It plays an essential role in cellular metabolism, energy production, and DNA repair mechanisms. As a central molecule in redox reactions, NAD+ exists in two forms: the oxidized form (NAD+) and the reduced form (NADH). The ratio between these two forms is critical for cellular function and metabolic health.

Key Research Interests:

• Cellular energy metabolism and bioenergetics

• Redox biology and electron transfer mechanisms

• Sirtuin enzyme activation and regulation studies

• DNA repair pathways (PARP enzyme research)

• Mitochondrial function and aging biology

• Neurodegenerative disease models

Researchers utilize NAD+ in controlled laboratory settings to study fundamental cellular processes including energy metabolism, DNA repair mechanisms, and calcium signaling pathways. The coenzyme is of particular interest to investigators focused on metabolic disorders, neurodegenerative diseases, and the basic biology of aging.

The Significance of NAD+ in Cellular Biology:
NAD+ is one of the most abundant and critical molecules in cellular metabolism. It serves two primary functions in cells:

Age‑Related NAD+ Decline:
NAD+ levels decline significantly with age, a phenomenon linked to reduced cellular repair capacity, mitochondrial dysfunction, and increased susceptibility to age‑related conditions. This age‑related decline makes NAD+ metabolism a highly active area of basic research in gerontology and regenerative medicine.

Third‑Party Testing and Quality Assurance

Every batch of Premium NAD+ 500MG undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third‑party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high‑performance liquid chromatography (HPLC)

• Batch‑to‑batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity of ≥99% as confirmed by HPLC, NAD+ meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Chemical and Physical Properties

Molecular Characteristics

NAD+ is a dinucleotide consisting of two nucleotides joined by their phosphate groups. One nucleotide contains an adenine base, while the other contains a nicotinamide base.

Detailed Molecular Information:

• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂

• Molecular Mass: 663.43 daltons

• CAS Number: 53-84-9

• EINECS Number: 200-184-4

• Purity: ≥99% (HPLC)

• Appearance: White to off‑white lyophilized powder

• Solubility: Freely soluble in water

Structural Features:

• Contains a nicotinamide ring that can accept an electron and a proton to become NADH

• Contains an adenine nucleotide

• Linked by two phosphate groups (pyrophosphate linkage)

• Positively charged nicotinamide ring

Physical Form and Storage

The product is supplied as a lyophilized (freeze‑dried) powder within a sterile, sealed vial. Lyophilization preserves the chemical integrity of the coenzyme by removing water content while maintaining the compound’s structure.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long‑term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze‑thaw cycles once reconstituted

Stability Considerations:
NAD+ is hygroscopic (absorbs moisture from the air) and can degrade when exposed to humidity, heat, or light. Proper storage is essential to maintain purity. Once reconstituted in aqueous solution, NAD+ is less stable than in lyophilized form and should be used promptly or stored frozen in aliquots. Short‑term storage (weeks) of reconstituted solution can be at 2‑8°C under sterile conditions.

Intended Use and Regulatory Status

Research Use Only

NAD+ is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating cellular metabolism and signaling

• Enzyme assays — studying NAD+‑dependent enzymes (sirtuins, PARPs, CD38)

• Biochemical research — redox reaction and electron transport studies

• Aging research — investigating NAD+ decline and cellular senescence

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into cellular metabolism

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

NAD+ should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for NAD+ 500MG:
For researchers needing a 100mg/mL working solution, add 5mL of sterile solvent to the 500mg vial. For a 50mg/mL solution, add 10mL of solvent. For a 25mg/mL solution, add 20mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
NAD+ is freely soluble in water. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate‑buffered saline (PBS) at neutral to slightly alkaline pH. NAD+ solutions are typically stable at neutral to slightly alkaline pH but degrade more rapidly under acidic conditions.

Disposal Considerations

Dispose of NAD+ and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

NAD+ is used in various laboratory research contexts, spanning biochemistry, cell biology, and molecular biology. Typical applications include:

Redox Biology and Metabolism Research:
NAD+ is central to cellular energy metabolism. Researchers use NAD+ to study:

• Glycolysis and the conversion of glucose to pyruvate

• The Krebs cycle (citric acid cycle) and oxidative metabolism

• Fatty acid beta‑oxidation pathways

• Electron transport chain function

• Lactate dehydrogenase (LDH) assays

• Alcohol dehydrogenase studies

Sirtuin and PARP Enzyme Research:
NAD+ is a critical substrate for NAD+‑dependent enzymes. Common research applications include:

• Sirtuin (SIRT1‑7) enzyme activity assays

• PARP (poly‑ADP‑ribose polymerase) activity studies

• CD38 and CD157 enzyme characterization

• NADase activity measurements

• Deacetylation and ADP‑ribosylation assays

Aging and Longevity Research:
The decline in NAD+ levels with age is a highly active area of basic research. NAD+ 500MG is used to study:

• Age‑related changes in cellular metabolism

• Sirtuin‑mediated pathways in aging models

• DNA repair mechanisms and genomic stability

• Cellular senescence markers and pathways

• Mitochondrial dysfunction and stem cell aging

Neurobiology Research:
NAD+ plays important roles in neuronal function and survival. Researchers use NAD+ 500MG to study:

• Neuronal energy metabolism

• Axonal degeneration mechanisms

• Neuroprotective signaling pathways

• Calcium homeostasis in neurons

• Neurodegenerative disease models (Alzheimer’s, Parkinson’s)

Cell Culture Studies:
NAD+ is used in controlled cell culture systems to investigate:

• Cellular responses to NAD+ modulation

• Metabolic flux and energy homeostasis

• Cell viability and proliferation

• Oxidative stress responses

• Mitochondrial function

Enzyme Kinetics and Assay Development:
NAD+ is used as a substrate and standard in analytical applications:

• HPLC method development and validation

• Mass spectrometry calibration standards

• Enzymatic assay validation

• Metabolomics research

Researchers should design their experimental protocols based on peer‑reviewed literature and established laboratory practices specific to NAD+ 500MG biochemistry and coenzyme research.

What Makes This Product Different

Essential Cellular Coenzyme

NAD+NAD+ 500MGcentral role in metabolism and cellular signaling makes it a critical reagent for a wide range of biological research applications.

High‑Capacity 500MG Vials for Large‑Scale Research

The 500mg vial size is specifically designed for researchers conducting large‑scale experiments, high‑throughput enzyme assays, extended time‑course studies, or protocols requiring substantial coenzyme quantities. This high capacity reduces the number of vials needed for extensive research programs.

Well‑Characterized Research History

NAD+ has been extensively studied in clinical and preclinical research. Multiple clinical trials have established the safety and bioavailability profiles of NAD+ precursors, providing researchers with a robust foundation for experimental design.

Rigorous Quality Control

Unlike research chemicals sold without verification, NAD+ comes with documented third‑party testing for every batch, including HPLC purity analysis and mass spectrometry identity confirmation.

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and synonyms. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Batch Traceability

Each package of NAD+ includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of NAD+?

Each batch is tested to verify purity of ≥99% via HPLC, with identity confirmed by mass spectrometry. Individual certificates of analysis are available upon request. NAD+ 500MG

What is the difference between NAD+ and NADH?

NAD+ is the oxidized form of nicotinamide adenine dinucleotide, while NADH is the reduced form. The two forms differ by the addition of a hydride ion (H⁻) to the nicotinamide ring. Researchers use both forms depending on whether they need to study oxidation or reduction reactions.

What is the significance of the CAS number 53-84-9?

The CAS number is a unique identifier for NAD+ 500MG, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing NAD+ 500MG in publications.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. NAD+ 500MG is hygroscopic, so keeping the vial tightly sealed and desiccated is particularly important. The lyophilized powder has a shelf life of up to 24 months when stored properly. NAD+ 500MG

What solvent should be used for reconstitution?

NAD+ is freely soluble in water. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral to slightly alkaline pH. Avoid acidic solvents (below pH 5.0) which accelerate degradation. NAD+ 500MG

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22‑micron filter may be appropriate. NAD+ 500MG

What research applications is NAD+ used for?

NAD+ 500MG is studied in redox biology, cellular metabolism, sirtuin and PARP enzyme research, aging biology, neurobiology, and cell culture investigations in laboratory research settings.

How stable is NAD+ in solution?

NAD+ 500MG is less stable in aqueous solution than in lyophilized form. For optimal stability, reconstituted solutions should be used promptly or aliquoted and frozen at -80°C. Short‑term storage (up to 2 weeks) at 2‑8°C is acceptable under sterile conditions.

Can NAD+ be used in combination with other research compounds?

Researchers should determine compatibility based on their specific experimental design. NAD+ 500MG is often used in enzyme assays with specific substrates and cofactors. Standard laboratory practice includes testing interactions in control experiments before full‑scale implementation.

Final Notes for Laboratory Researchers

Premium NAD+ 500MG represents a high‑purity research tool for qualified professionals conducting legitimate scientific investigations into cellular metabolism, redox biology, enzyme kinetics, aging mechanisms, and related fields. With documented third‑party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers. NAD+ 500MG

The 500mg vial size is specifically optimized for large‑scale research applications requiring substantial coenzyme quantities, including high‑throughput enzyme assays, extended time‑course studies, and protocols demanding consistent batch material across numerous experiments. As an essential cellular coenzyme with well‑characterized research history, NAD+ 500MG offers fundamental research opportunities in metabolism, aging, and cellular signaling.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training. NAD+ 500MG