Premium Ipamorelin Research Peptide — High-Purity Selective GHSR Agonist for Laboratory Use

Product Overview

Premium Ipamorelin is a research‑grade synthetic pentapeptide and a selective agonist of the ghrelin receptor (growth hormone secretagogue receptor, GHSR). It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 5mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 5mg per vial, 10 vials per pack

• Form: Lyophilized (freeze‑dried) powder

• Purity: ≥99% verified by third‑party testing

• Molecular Formula: C₃₈H₄₉N₉O₅

• Molecular Mass: 711.9

• CAS Number: 170851-70-4

• Sequence: Aib-His-D-2-Nal-D-Phe-Lys-NH₂

• Synonyms: NNC-26-0161, IPAM

• Research Use Only: Not for human or animal consumption

Understanding Ipamorelin: The Research Context

Ipamorelin is a synthetic pentapeptide belonging to the growth hormone secretagogue (GHS) class of compounds. It is a potent and selective agonist of the ghrelin receptor (GHS‑R1a), which stimulates the release of growth hormone (GH) from the pituitary gland. As a third‑generation GHRP (growth hormone‑releasing peptide), Ipamorelin is a more refined research tool compared to its predecessors GHRP‑6 and GHRP‑2.

Key Research Interests:

• GHSR (ghrelin receptor) activation and Gαq-coupled signaling pathways

• Growth hormone pulse amplitude modulation in vitro and in vivo

• Selective GH axis activation without elevation of prolactin or cortisol

• Gastric motility and gastrointestinal transit research

• Pituitary somatotroph function and cellular signaling

Researchers utilize Ipamorelin in controlled laboratory settings to study the selective activation of the ghrelin receptor and its downstream signaling consequences. The peptide is of particular interest to investigators focused on endocrinology, gastroenterology, and the fundamental mechanisms of GHSR pharmacology.

The Significance of GHSR Selectivity:
Early growth hormone secretagogues, while effective at stimulating GH release, were associated with increases in other pituitary hormones such as cortisol and prolactin. Ipamorelin was developed to overcome this limitation. Unlike GHRP‑6 and GHRP‑2, Ipamorelin demonstrates high selectivity for GH release with minimal effects on ACTH, cortisol, or prolactin levels at any dose. This selectivity makes Ipamorelin a valuable research tool for investigators studying isolated GH axis activation without the confounding effects of broader hormonal changes.

Ipamorelin in the Growth Hormone Secretagogue Family

Ipamorelin belongs to the growth hormone secretagogue (GHS) family of peptides, which includes several compounds with distinct properties.

Research Significance of Ipamorelin’s Selectivity:
The selectivity profile of Ipamorelin allows researchers to:

• Study growth hormone release with minimal interference from other hormonal pathways

• Isolate GHSR‑mediated effects from broader pituitary activation

• Investigate GH axis regulation without confounding cortisol or prolactin changes

Third‑Party Testing and Quality Assurance

Every batch of Premium Ipamorelin undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third‑party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high‑performance liquid chromatography (HPLC)

• Batch‑to‑batch consistency checks

• Endotoxin testing (<0.05 EU/mg) confirmed by LAL‑validated methods

• Residual solvent analysis to confirm complete lyophilization

With a verified purity of ≥99% as confirmed by HPLC, Ipamorelin meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Chemical and Physical Properties

Molecular Characteristics

Ipamorelin is a synthetic pentapeptide (5 amino acids) with a unique structure that includes non‑standard amino acids, conferring enhanced stability and receptor selectivity.

Detailed Molecular Information:

• Molecular Formula: C₃₈H₄₉N₉O₅

• Molecular Mass: 711.9 g/mol

• CAS Number: 170851-70-4

• Sequence: Aib-His-D-2-Nal-D-Phe-Lys-NH₂

• Purity: ≥99% (HPLC)

• Appearance: White to off‑white lyophilized powder

Amino Acid Breakdown and Properties:

Structural Features:

• Linear pentapeptide (5 amino acids)

• Contains non‑standard amino acids Aib and D-2-Nal

• Contains D-Phe (D‑isomer of phenylalanine)

• Positively charged lysine residue at the C‑terminus

• Amidated C‑terminus (NH₂)

• Enhanced enzymatic stability due to D‑amino acids

The presence of multiple non‑standard amino acids (Aib, D-2-Nal, D-Phe) distinguishes Ipamorelin from naturally occurring peptides and contributes to its resistance to enzymatic degradation, making it more stable in laboratory settings.

Mechanism of Action

Ipamorelin acts as a selective agonist of the growth hormone secretagogue receptor (GHS‑R1a), which is the same receptor targeted by the hunger hormone ghrelin.

Research‑Characterized Binding and Signaling:

Signaling Pathway:
Ipamorelin binds to and activates GHS‑R1a, a Gαq‑coupled GPCR on pituitary somatotrophs. Activation triggers phospholipase C, leading to IP₃ production and calcium release, which in turn stimulates GH secretion.

Physical Form and Storage

The product is supplied as a lyophilized (freeze‑dried) powder within a sterile, sealed vial. The powder appears as a white to off‑white substance.

Recommended Storage Conditions:

• Lyophilized powder: Store at -20°C (-4°F) or below for long‑term preservation (stable for up to 3 years)

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze‑thaw cycles once reconstituted

Reconstituted Solution Storage:
Once reconstituted with an appropriate sterile solvent, the solution should be stored at 2‑8°C (refrigerated) and used within 2‑7 days. For extended storage after reconstitution, freezing at -80°C in aliquots is recommended, ideally with the addition of a carrier protein (0.1% HSA or BSA) to enhance stability.

Intended Use and Regulatory Status

Research Use Only

Ipamorelin is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating GHSR signaling in pituitary cell lines

• Receptor binding assays — studying GHS‑R1a interactions and selectivity

• Endocrine research — investigating GH axis regulation and feedback loops

• Gastrointestinal research — studying gastric motility and transit models

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into GHSR agonist structure and function

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

Ipamorelin should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for Ipamorelin 5MG:
For researchers needing a 5mg/mL working solution, add 1mL of sterile solvent to the 5mg vial. For a 2.5mg/mL solution, add 2mL of solvent. For a 1mg/mL solution, add 5mL of solvent. For a 0.5mg/mL solution, add 10mL of solvent. It is recommended to reconstitute in sterile 18MΩ·cm water at a concentration not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. Always verify calculations before proceeding with experiments.

Solvent Considerations:
Ipamorelin is water‑soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate‑buffered saline (PBS) at neutral pH.

Disposal Considerations

Dispose of Ipamorelin and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

Ipamorelin is used in various laboratory research contexts, primarily focused on GHSR pharmacology, GH axis regulation, and gastrointestinal research. Typical applications include:

GHSR Signaling and Receptor Studies:
The primary research interest in Ipamorelin revolves around its selective activation of GHS‑R1a. Researchers use Ipamorelin to study:

• Receptor binding affinity and kinetics (Ki = 63.4 nM for GHS‑R1a)

• Gαq protein coupling and phospholipase C activation

• IP₃ accumulation and calcium mobilization

• Structure‑activity relationships (SAR) of GHSR agonists

• Receptor desensitization and internalization pathways

Growth Hormone Pulse Amplitude Research:
Ipamorelin specifically amplifies GH pulse amplitude without affecting baseline pulsatile frequency. Research applications include:

• Concentration‑dependent GH release (EC₅₀ = 1.3 nM in vitro; ED₅₀ = 80 nmol/kg in rats)

• GH pulse generation mechanisms

• Somatotroph responsiveness and regulation

• GH axis feedback studies

Gastrointestinal Motility Research:
Ipamorelin has been studied for its effects on gastric emptying. Research applications include:

• Postoperative ileus models (0.014 or 0.14 μmol/kg)

• Gastrointestinal transit rate investigations

• Ghrelin receptor‑mediated gastric motility pathways

• Enteric nervous system signaling

Comparative GHSR Pharmacology:
Ipamorelin’s selectivity makes it valuable for:

• Comparing selective vs. non‑selective GHSR activation

• Isolating GH‑specific effects from broader hormonal changes

• Studying the functional significance of cortisol/prolactin‑sparing pathways

GPCR Signaling Research:
GHS‑R1a is a prototypical Gαq‑coupled GPCR. Ipamorelin serves as a research tool for:

• G protein coupling specificity studies

• β‑arrestin recruitment and trafficking assays

• MAPK/ERK pathway activation investigations

• cAMP/PKA and kinase cascade mapping

Pituitary Cell Culture Studies:
Researchers use Ipamorelin in controlled pituitary cell culture systems to investigate:

• Somatotroph function and GH secretion dynamics

• Pituitary receptor expression and regulation

• Calcium‑dependent signaling pathways

• Proliferation and differentiation of GH‑producing cells

In Vivo Research Models:
Preclinical research has utilized Ipamorelin in animal models with observations including:

• Increased plasma GH levels in rats (ED₅₀ = 80 nmol/kg) and pigs (ED₅₀ = 2.3 nmol/kg)

• Enhanced longitudinal bone growth in rats (18‑450 μg/animal)

• Increased gastric emptying in a rat model of postoperative ileus

Researchers should design their experimental protocols based on peer‑reviewed literature and established laboratory practices specific to GHSR agonists.

What Makes This Product Different

High Selectivity for GHSR

Unlike earlier growth hormone secretagogues such as GHRP‑6 and GHRP‑2, which are associated with increases in cortisol and prolactin, Ipamorelin demonstrates high selectivity for GH release without affecting ACTH or cortisol levels. This selectivity makes Ipamorelin a valuable research tool for investigators studying isolated GH axis activation without confounding hormonal effects.

Unique Non‑Standard Amino Acid Composition

Ipamorelin contains three non‑standard amino acids:

• Aib (aminoisobutyric acid): An achiral, non‑standard residue that introduces structural rigidity and enhances stability

• D-2-Nal (D-2-naphthylalanine): A synthetic amino acid with a bulky naphthyl side chain that contributes to high receptor binding affinity

• D-Phe (D-phenylalanine): The D‑isomer of phenylalanine that confers resistance to proteolytic degradation

This unique composition distinguishes Ipamorelin from naturally occurring peptides and contributes to its stability and receptor binding properties.

Enhanced Enzymatic Stability

The presence of D‑amino acids and non‑standard residues makes Ipamorelin more resistant to proteolytic degradation compared to all‑L‑amino acid peptides. This enhanced stability is valuable for laboratory research applications where peptide integrity over time is important.

No Significant Appetite Stimulation

Unlike the natural ghrelin hormone and some other GHSR agonists, Ipamorelin does not stimulate appetite or promote hunger. This property allows researchers to study GHSR‑mediated GH release without the confounding metabolic effects of increased food intake.

Rigorous Quality Control

Unlike research chemicals sold without verification, Premium Ipamorelin comes with documented third‑party testing for every batch, including HPLC purity analysis, mass spectrometry identity confirmation, and endotoxin testing (<0.05 EU/mg).

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and amino acid sequence. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Appropriate Packaging for Research Use

The sterile, sealed vial packaging protects the lyophilized powder from environmental contaminants. The 5mg concentration is optimized for laboratory research applications requiring precise handling.

Batch Traceability

Each package of Ipamorelin includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of Premium Ipamorelin?

Each batch is tested to verify purity of ≥99% via HPLC, with identity confirmed by mass spectrometry. Endotoxin levels are confirmed at <0.05 EU/mg via LAL‑validated methods. Individual certificates of analysis are available upon request.

What is the significance of the CAS number 170851-70-4?

The CAS number is a unique identifier for Ipamorelin, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications.

What is the amino acid sequence of Ipamorelin?

The sequence is Aib‑His‑D‑2‑Nal‑D‑Phe‑Lys‑NH₂, where Aib represents aminoisobutyric acid, D‑2‑Nal represents D‑2‑naphthylalanine, and D‑Phe represents D‑phenylalanine.

What is the mechanism of action of Ipamorelin?

Ipamorelin is a selective agonist of the ghrelin receptor (GHS‑R1a). Activation of this Gαq‑coupled GPCR triggers phospholipase C, leading to IP₃ accumulation, calcium release, and downstream GH secretion from pituitary somatotrophs.

How does Ipamorelin differ from GHRP‑6 and GHRP‑2?

Unlike GHRP‑6 and GHRP‑2, which cause increases in ACTH and cortisol levels, Ipamorelin demonstrates high selectivity for GH release without affecting other pituitary hormones. Ipamorelin also has a longer plasma half‑life and is moderately resistant to metabolism, with 60‑80% of an administered dose recoverable as intact peptide.

What is the difference between Ipamorelin and natural ghrelin?

Both target GHS‑R1a, but ghrelin strongly stimulates appetite and gastric emptying. Ipamorelin, as a synthetic analog, shares the GH‑releasing properties of ghrelin but does not promote hunger.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Lyophilized powder is stable for up to 3 years at -20°C.

What solvent should be used for reconstitution?

Ipamorelin is water‑soluble. It is recommended to reconstitute in sterile 18MΩ·cm water at a concentration of at least 100 µg/mL, which can then be further diluted to other aqueous solutions. Common options include bacteriostatic water or PBS at neutral pH.

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. Endotoxin levels are confirmed at <0.05 EU/mg. For cell culture applications, additional filtration using a 0.22‑micron filter may be appropriate.

What research applications is Ipamorelin used for?

Ipamorelin is studied in GHSR signaling research, GH axis regulation, GPCR pharmacology, gastric motility models, and endocrine stress feedback research in laboratory cell culture and research models.

Can Ipamorelin be used in combination with other research peptides?

Yes. Ipamorelin is frequently combined with GHRH analogs such as CJC‑1295 (No DAC) or Modified GRF (1‑29) to study dual‑pathway GH axis activation. The GHRH analog primarily influences GH pulse frequency, while Ipamorelin amplifies the amplitude of GH pulses. Researchers should determine compatibility based on their specific experimental design.

Final Notes for Laboratory Researchers

Premium Ipamorelin represents a high‑purity research tool for qualified professionals conducting legitimate scientific investigations into GHSR signaling, GH axis regulation, gastrointestinal motility, and related endocrinological fields. With documented third‑party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 5mg vial size is optimized for cell culture studies, receptor binding assays, and dose‑response experiments requiring precise handling. As a selective ghrelin receptor agonist with well‑characterized Gαq‑coupled GPCR pharmacology, Ipamorelin offers unique research opportunities for studying GH pulse dynamics, GHSR signaling pathways, and isolated GH axis activation.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.