MT-2

Premium MT-2 Research Peptide — High-Purity Cyclic Melanocortin Analog for Laboratory Use

Product Overview

Premium MT‑2 (Melanotan‑II) is a research‑grade synthetic cyclic peptide analog of alpha‑melanocyte stimulating hormone (α‑MSH). It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 10mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 10mg per vial, 10 vials per pack

• Form: Lyophilized (freeze‑dried) powder

• Purity: ≥98% verified by third‑party testing

• Molecular Formula: C₅₀H₆₉N₁₅O₉

• Molecular Mass: 1024.23

• CAS Number: 121062-08-6

• Sequence: Ac‑Nle‑cyclo[Asp‑His‑D‑Phe‑Arg‑Trp‑Lys]‑NH₂

• Synonyms: Melanotan‑II, MT‑II, MT2

• Research Use Only: Not for human or animal consumption

Understanding MT‑2: The Research Context

MT‑2 (Melanotan‑II) is a synthetic cyclic heptapeptide developed as an analog of alpha‑melanocyte stimulating hormone (α‑MSH). Unlike its linear counterpart Melanotan‑I (MT‑1), MT‑2 features a cyclic structure created by a lactam bridge between aspartic acid and lysine residues. This cyclization confers enhanced metabolic stability and restricted conformational flexibility, making MT‑2 a valuable research tool for studying melanocortin receptor biology.

Key Research Interests:

• Melanocortin receptor (MC1R, MC3R, MC4R, MC5R) binding and selectivity

• G‑protein coupled receptor (GPCR) signaling pathways

• Cyclic peptide structure‑activity relationships

• Pigmentation (melanogenesis) pathway research

• Appetite regulation and energy homeostasis studies

• In vitro cell culture investigations

Researchers utilize MT‑2 in controlled laboratory settings to study melanocortin receptor pharmacology, pigmentation mechanisms, and metabolic regulation. The peptide is of particular interest to investigators focused on MC1R signaling, melanogenesis, and the broader melanocortin receptor family.

The Significance of the Cyclic Structure:
MT‑2 was designed with a lactam bridge between Asp⁵ and Lys¹⁰ (positions in the cyclic sequence), creating a rigid, cyclic conformation. This structural feature was developed based on NMR and computer modeling that suggested a salt bridge exists between Glu⁵ and Lys¹¹ in natural α‑MSH. The cyclic structure provides:

• Greater metabolic stability (resistance to proteases)

• Restricted conformational flexibility

• Enhanced receptor binding affinity and selectivity

The Melanocortin Receptor Family

The melanocortin system consists of five known G‑protein coupled receptors (MC1R‑MC5R). MT‑2 acts as a non‑selective agonist at multiple receptor subtypes, making it a broad‑spectrum research tool.

Research Significance of Non‑Selectivity:
MT‑2’s activity at multiple receptors allows researchers to study the integrated functions of the melanocortin system. However, for receptor‑specific studies, more selective analogs may be required. Investigators should consult the literature to determine if MT‑2 or a selective analog (e.g., MT‑1 for MC1R) is appropriate for their experimental design.

Third‑Party Testing and Quality Assurance

Every batch of Premium MT‑2 undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third‑party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high‑performance liquid chromatography (HPLC)

• Batch‑to‑batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity of ≥98% as confirmed by HPLC, MT‑2 meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Chemical and Physical Properties

Molecular Characteristics

MT‑2 is a synthetic cyclic heptapeptide with a lactam bridge between aspartic acid (Asp) and lysine (Lys) residues.

Detailed Molecular Information:

• Molecular Formula: C₅₀H₆₉N₁₅O₉

• Molecular Mass: 1024.23 daltons

• CAS Number: 121062-08-6

• Sequence: Ac‑Nle‑cyclo[Asp‑His‑D‑Phe‑Arg‑Trp‑Lys]‑NH₂

• Purity: ≥98% (HPLC)

• Appearance: White to off‑white lyophilized powder

Structural Features:

• Cyclic heptapeptide (7 amino acids in the ring)

• Contains D‑Phe (D‑phenylalanine) instead of L‑Phe

• Acetylated N‑terminus

• Amidated C‑terminus

• Nle (norleucine) substitution for methionine

• Lactam bridge between Asp and Lys

• Water‑soluble

The presence of D‑Phe (the D‑isomer of phenylalanine) and the cyclic lactam bridge distinguish MT‑2 from naturally occurring linear peptides. These features confer enhanced resistance to proteolytic degradation and a defined bioactive conformation.

Physical Form and Storage

The product is supplied as a lyophilized (freeze‑dried) powder within a sterile, sealed vial. Lyophilization preserves the chemical integrity of the peptide by removing water content while maintaining the compound’s structure.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long‑term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze‑thaw cycles once reconstituted

Stability Considerations:
Lyophilized MT‑2 is stable at room temperature for up to 3 weeks but should be stored desiccated below -18°C for long‑term preservation. Upon reconstitution, MT‑2 solutions can be stored at 4°C for 2‑7 days. For extended storage after reconstitution, freezing below -18°C is recommended, preferably with the addition of a carrier protein such as 0.1% HSA or BSA to enhance stability.

Intended Use and Regulatory Status

Research Use Only

MT‑2 is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating peptide interactions with cellular systems

• Receptor binding assays — studying melanocortin receptor interactions

• Melanogenesis research — pigmentation pathway investigations

• GPCR signaling studies — examining cAMP and other second messengers

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into peptide structure and function

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

MT‑2 should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for MT‑2 10MG:
For researchers needing a 5mg/mL working solution, add 2mL of sterile solvent to the 10mg vial. For a 10mg/mL solution, add 1mL of solvent. For a 2mg/mL solution, add 5mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
It is recommended to reconstitute lyophilized MT‑2 in sterile 18 MΩ·cm water at a concentration not less than 100 µg/mL, which can then be further diluted with other aqueous solutions. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate‑buffered saline (PBS) at neutral pH. MT-2

Disposal Considerations

Dispose of MT‑2 and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines. MT-2

Research Applications

MT‑2 is used in various laboratory research contexts, primarily focused on melanocortin receptor pharmacology, pigmentation, and metabolic regulation. Typical applications include:

Melanocortin Receptor Binding Studies:
The primary research interest in MT‑2 revolves around its interaction with multiple melanocortin receptor subtypes. Researchers use MT‑2 to study:

• Receptor binding affinity and kinetics at MC1R, MC3R, MC4R, and MC5R

• Competitive binding assays with endogenous melanocortin ligands (α‑MSH, β‑MSH, ACTH)

• Structure‑activity relationships (SAR) of cyclic melanocortin agonists

• Receptor binding domain mapping

G‑Protein Coupled Receptor (GPCR) Signaling:
Melanocortin receptors are prototypical GPCRs. MT‑2 serves as a research tool for:

• GPCR activation mechanisms and conformational changes

• Second messenger signaling (cAMP, IP₃, calcium)

• Gs protein coupling and adenylyl cyclase activation

• β‑arrestin recruitment and receptor internalization

Melanogenesis and Pigmentation Research:
Through MC1R activation, MT‑2 is used to study:

• Melanin synthesis pathways (eumelanin vs. pheomelanin)

• Melanocyte biology and differentiation

• Tyrosinase activity and gene expression

• Pigmentation regulation mechanisms in cell culture

Appetite and Energy Homeostasis Research:
Due to its activity at MC3R and MC4R (central regulators of feeding), MT‑2 is used in:

• Energy balance studies

• Food intake regulation models

• Hypothalamic signaling pathways

• Metabolic syndrome research

Cell Culture Investigations:
MT‑2 is used in controlled cell culture systems to investigate:

• Melanocortin receptor expression and regulation

• Cellular responses to receptor activation

• cAMP‑responsive element (CRE)‑mediated transcription

• Functional consequences of melanocortin signaling

Researchers should design their experimental protocols based on peer‑reviewed literature and established laboratory practices specific to melanocortin peptides.

What Makes This Product Different

Unique Cyclic Heptapeptide Structure

Unlike linear melanocortin analogs, MT‑2 has a cyclic structure stabilized by a lactam bridge. This cyclic conformation provides greater structural rigidity, enhanced metabolic stability, and resistance to enzymatic degradation, making it a valuable research tool for studying constrained peptide conformations. MT-2

Broad Melanocortin Receptor Activity

MT‑2 is a non‑selective agonist at multiple melanocortin receptor subtypes (MC1R, MC3R, MC4R, MC5R). This receptor profile distinguishes MT‑2 from more selective analogs and makes it useful for researchers studying the broader melanocortin system rather than individual receptor subtypes.

D‑Amino Acid Incorporation

The presence of D‑Phe (the D‑isomer of phenylalanine) distinguishes MT‑2 from naturally occurring peptides, which typically contain only L‑amino acids. This feature enhances proteolytic stability and allows structure‑activity studies of stereochemical effects. MT-2

Rigorous Quality Control

Unlike research chemicals sold without verification, MT‑2 comes with documented third‑party testing for every batch, including HPLC purity analysis and mass spectrometry identity confirmation.

Batch Traceability

Each package of MT‑2 includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation. MT-2

Frequently Asked Questions for Researchers

What is the purity level of MT‑2?

Each batch is tested to verify purity of ≥98% via HPLC, with identity confirmed by mass spectrometry. Individual certificates of analysis are available upon request.

What is the significance of the CAS number 121062-08-6?

The CAS number is a unique identifier for MT‑2 (Melanotan‑II), allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications. MT-2

What is the amino acid sequence of MT‑2?

The sequence is Ac‑Nle‑cyclo[Asp‑His‑D‑Phe‑Arg‑Trp‑Lys]‑NH₂, where Nle represents norleucine, D‑Phe represents D‑phenylalanine, and the cyclic structure is formed by a lactam bridge between Asp and Lys. MT-2

How does MT‑2 differ from MT‑1 (Melanotan‑I)?

MT‑2 is a cyclic heptapeptide with a lactam bridge, making it structurally constrained and more stable than MT‑1, which is a linear 13‑amino acid peptide. MT‑2 is also less receptor‑selective, acting at MC1R, MC3R, MC4R, and MC5R, whereas MT‑1 is primarily MC1R‑selective. 

What does the cyclic structure mean for research applications?

The cyclic structure restricts conformational flexibility, providing insights into the bioactive conformation required for receptor binding and activation. This makes MT‑2 valuable for structure‑activity relationship (SAR) studies of melanocortin receptors. MT-2

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Lyophilized MT‑2 is stable at room temperature for up to 3 weeks but should be stored desiccated below -18°C for long‑term preservation. MT-2

What solvent should be used for reconstitution?

It is recommended to reconstitute lyophilized MT‑2 in sterile 18 MΩ·cm water at a concentration not less than 100 µg/mL, then further dilute with aqueous buffers as needed. MT-2

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22‑micron filter may be appropriate.

What research applications is MT‑2 used for?

MT‑2 is studied in melanocortin receptor binding, GPCR signaling, melanogenesis (pigmentation), appetite and energy homeostasis, and structure‑activity relationship (SAR) analysis in laboratory cell culture and research models.

Can MT‑2 be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. Standard laboratory practice includes testing interactions in control experiments before full‑scale implementation.

Final Notes for Laboratory Researchers

Premium MT‑2 represents a high‑purity research tool for qualified professionals conducting legitimate scientific investigations into melanocortin receptor biology, GPCR signaling, melanogenesis, energy homeostasis, and related pharmacological fields. With documented third‑party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers. v

The 10mg vial size is optimized for receptor binding assays, cell culture studies, and dose‑response experiments. As a cyclic peptide with D‑amino acid content, MT‑2 offers unique research opportunities for studying constrained peptide conformations and stereochemical effects on melanocortin receptor binding. MT-2

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training. MT-2