Premium SEMAX Research Peptide — High-Purity ACTH(4-10) Analog for Laboratory Use

Product Overview

Premium SEMAX is a research‑grade synthetic heptapeptide derived from the N‑terminal fragment (4‑10) of adrenocorticotropic hormone (ACTH). It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 10mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 10mg per vial, 10 vials per pack

• Form: Lyophilized (freeze‑dried) powder

• Purity: ≥98% verified by third‑party testing

• Molecular Formula: C₃₇H₅₁N₉O₁₀S

• Molecular Mass: 813.92

• CAS Number: 80714-61-0

• Sequence: Met‑Glu‑His‑Phe‑Pro‑Gly‑Pro (MEHFPGP)

• Synonyms: ACTH (4-10) analog, Semax

• Research Use Only: Not for human or animal consumption

Understanding SEMAX: The Research Context

SEMAX is a synthetic research peptide that belongs to the family of ACTH(4‑10) analogs. The parent molecule, ACTH(4‑10) (Met‑Glu‑His‑Phe‑Arg‑Trp‑Gly), is a fragment of adrenocorticotropic hormone known to influence learning and memory processes in laboratory models. SEMAX is a stabilized analog of this fragment, with the C‑terminus modified by the addition of the tripeptide Pro‑Gly‑Pro. This modification provides markedly greater metabolic stability compared to the native sequence, making it more suitable for extended research protocols.

Key Research Interests:

• Nootropic (cognitive enhancement) mechanisms

• Neurotrophin synthesis (BDNF) and signaling

• Dopaminergic and serotonergic system modulation

• Neuroprotection and cellular repair pathways

• In vitro cell culture and neuropharmacology

Researchers utilize SEMAX in controlled laboratory settings to study the molecular pathways underlying learning, memory, and neural plasticity. The peptide is of particular interest to investigators focused on neuroscience, neuropharmacology, and the development of stable ACTH fragments for basic research.

The Significance of ACTH(4‑10) Research:
ACTH(4‑10) is a naturally occurring fragment of adrenocorticotropic hormone that has been shown to affect behavior, learning, and memory in animal models. However, it is rapidly degraded by enzymes, limiting its utility in laboratory research. SEMAX was designed to overcome this limitation:

• Enhanced stability: The Pro‑Gly‑Pro tail protects the peptide from enzymatic breakdown

• Retained bioactivity: The core ACTH(4‑7) sequence (Met‑Glu‑His‑Phe) is preserved

• Selective activity: Focuses on neurotrophic and neurotransmitter effects without hormonal side effects

Third‑Party Testing and Quality Assurance

Every batch of Premium SEMAX undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third‑party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high‑performance liquid chromatography (HPLC)

• Batch‑to‑batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity of ≥98% as confirmed by HPLC, SEMAX meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. Batch‑specific Certificates of Analysis (CoA) are available upon request.

Chemical and Physical Properties

Molecular Characteristics

SEMAX is a synthetic heptapeptide (7 amino acids) with a specific sequence derived from ACTH(4‑10) plus a stabilizing Pro‑Gly‑Pro C‑terminal extension.

Detailed Molecular Information:

• Molecular Formula: C₃₇H₅₁N₉O₁₀S

• Molecular Mass: 813.92 daltons

• CAS Number: 80714-61-0

• Sequence: Met‑Glu‑His‑Phe‑Pro‑Gly‑Pro (MEHFPGP)

• Purity: ≥98% (HPLC)

• Appearance: White to off‑white lyophilized powder

Structural Features:

• Heptapeptide (7 amino acids)

• Contains methionine (sulfur‑containing)

• Includes histidine and phenylalanine (aromatic residues)

• C‑terminal Pro‑Gly‑Pro tail for stability

• No disulfide bonds (linear peptide)

The presence of sulfur in methionine can make the peptide susceptible to oxidation if not handled carefully, though the lyophilized form is stable when stored properly.

Physical Form and Storage

The product is supplied as a lyophilized (freeze‑dried) powder within a sterile, sealed vial. Lyophilization preserves the chemical integrity of the peptide by removing water content while maintaining the compound’s structure.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long‑term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze‑thaw cycles once reconstituted

Stability Considerations:
Due to the presence of methionine, minimize air exposure and avoid repeated opening of stock vials. For long‑term storage of reconstituted solutions, aliquot and freeze at -80°C.

Once reconstituted with an appropriate sterile solvent, the solution should be used according to laboratory protocols.

Intended Use and Regulatory Status

Research Use Only

SEMAX is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating neurotrophin synthesis and receptor signaling

• Neurotransmitter research — dopaminergic and serotonergic pathway modulation

• Neuroprotection studies — protection against oxidative and excitotoxic stress

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into ACTH(4-10) analog biology

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

SEMAX should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for SEMAX 10MG:
For researchers needing a 5mg/mL working solution, add 2mL of sterile solvent to the 10mg vial. For a 10mg/mL solution, add 1mL of solvent. For a 2mg/mL solution, add 5mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
SEMAX is water‑soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate‑buffered saline (PBS) at neutral pH.

Disposal Considerations

Dispose of SEMAX and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

SEMAX is used in various laboratory research contexts, primarily focused on neuroscience, neuropharmacology, and cellular signaling. Typical applications include:

Neurotrophin (BDNF) Research:
SEMAX has been shown to stimulate the synthesis of brain‑derived neurotrophic factor (BDNF), a key modulator of synaptic plasticity. Researchers use SEMAX to study:

• BDNF gene expression and protein production

• TrkB receptor activation and downstream signaling

• Synaptic potentiation and long‑term memory models

• Neuroprotective effects in cell culture

Dopaminergic and Serotonergic System Studies:
Research has demonstrated that SEMAX activates dopaminergic and serotonergic brain systems in laboratory models. Applications include:

• Dopamine release and turnover assays

• Serotonin metabolite measurements

• Monoamine receptor expression and sensitivity

• Behavioral pharmacology in model systems

Neuroprotection Research:
SEMAX has been investigated for its ability to protect neurons from various insults. Research applications include:

• Protection against glutamate‑induced excitotoxicity

• Reduction of oxidative stress in neuronal cultures

• Preservation of mitochondrial function under stress

• Anti‑inflammatory effects in glial cells

Cellular Signaling Investigations:
Researchers use SEMAX to study:

• PKA and CREB activation pathways

• Calcium signaling in neurons

• Neurotransmitter receptor trafficking

• Gene expression changes following peptide treatment

Cell Culture Models:
SEMAX is used in controlled cell culture systems to investigate:

• Neuronal survival and differentiation

• Neurite outgrowth and synaptogenesis

• Astrocyte activation and neurotrophin release

• Microglial inflammatory responses

Researchers should design their experimental protocols based on peer‑reviewed literature and established laboratory practices specific to ACTH(4‑10) analogs.

What Makes This Product Different

Stabilized ACTH(4‑10) Analog

Unlike the native ACTH(4‑10) fragment, which degrades rapidly, SEMAX incorporates a Pro‑Gly‑Pro C‑terminal extension that provides exceptional stability. This allows researchers to conduct extended time‑course experiments without peptide degradation.

Neurotrophin‑Modulating Properties

SEMAX has been shown to increase BDNF levels, a key regulator of synaptic plasticity and neuronal survival. This neurotrophin‑modulating activity distinguishes it from many other research peptides that lack such specific effects on neurotrophic signaling.

Well‑Characterized Neurotransmitter Effects

Research has demonstrated that SEMAX influences both dopaminergic and serotonergic systems, making it a valuable tool for studying monoamine pathways in the context of cognition and neuroprotection.

Rigorous Quality Control

Unlike research chemicals sold without verification, SEMAX comes with documented third‑party testing for every batch, including HPLC purity analysis and mass spectrometry identity confirmation.

Batch Traceability

Each package of SEMAX includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of SEMAX?

Each batch is tested to verify purity of ≥98% via HPLC, with identity confirmed by mass spectrometry. Individual certificates of analysis are available upon request.

What is the significance of the CAS number 80714-61-0?

The CAS number is a unique identifier for SEMAX, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications.

What is the amino acid sequence of SEMAX?

The sequence is Met‑Glu‑His‑Phe‑Pro‑Gly‑Pro (MEHFPGP).

How does SEMAX differ from native ACTH(4‑10)?

SEMAX is a stabilized analog of ACTH(4‑10) with a Pro‑Gly‑Pro tail that provides greater metabolic stability. The native sequence is rapidly degraded, while SEMAX is suitable for extended research protocols.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Due to the presence of methionine, minimize air exposure. Avoid repeated freeze-thaw cycles once reconstituted.

What solvent should be used for reconstitution?

SEMAX is water‑soluble. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral pH.

What research applications is SEMAX used for?

SEMAX is studied in neurotrophin (BDNF) research, dopaminergic and serotonergic systems, neuroprotection, and cellular signaling in laboratory cell culture and research models.

Can SEMAX be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. Standard laboratory practice includes testing interactions in control experiments before full‑scale implementation.

Final Notes for Laboratory Researchers

Premium SEMAX represents a high‑purity research tool for qualified professionals conducting legitimate scientific investigations into neurotrophin signaling, monoamine systems, neuroprotection, and related neuroscientific fields. With documented third‑party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 10mg vial size is optimized for cell culture studies, neuropharmacological assays, and dose‑response experiments. As a stabilized ACTH(4‑10) analog with well‑characterized neurotrophic properties, SEMAX offers unique research opportunities in the study of learning, memory, and neural plasticity at the molecular level.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.