GHK-Cu 100MG

Product Overview

Premium GHK‑Cu is a research‑grade naturally occurring copper‑binding tripeptide. It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 100 mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 100 mg per vial, 10 vials per pack

• Form: Lyophilized (freeze‑dried) powder

• Purity: ≥99% verified by third‑party testing

• Molecular Formula: C₁₄H₂₄CuN₆O₄

• Molecular Mass: 401.93 g/mol

• CAS Number: 49557‑75‑7

• Sequence: Gly‑His‑Lys (glycine‑histidine‑lysine)

• Synonyms: Copper tripeptide‑1, prezatide copper, GHK copper complex

• Research Use Only: Not for human or animal consumption

Understanding GHK‑Cu: The Research Context

GHK‑Cu is a naturally occurring copper‑binding tripeptide composed of three amino acids: glycine, histidine, and lysine. It was first isolated from human plasma in the 1970s and has since become one of the most extensively studied peptides in regenerative research.

Key Research Interests:

• Gene expression modulation and regulation

• Collagen and elastin synthesis stimulation

• Extracellular matrix (ECM) remodeling

• Wound healing and tissue repair mechanisms

• Antioxidant defense pathway activation

• In vitro cell culture investigations

Researchers utilize GHK‑Cu in controlled laboratory settings to study its role in gene regulation, tissue remodeling, and cellular signaling. The peptide is of particular interest to investigators focused on regenerative biology, extracellular matrix dynamics, and the fundamental mechanisms of tissue repair.

The Significance of GHK‑Cu in Human Biology:
GHK‑Cu is naturally present in human plasma at approximately 200 ng/mL in young adults. This level declines significantly with age, falling to around 80 ng/mL by age 60 and to approximately 10 ng/mL by age 80. This age‑related decline parallels reductions in healing capacity and tissue quality, making GHK‑Cu a molecule of significant research interest in aging biology and regenerative medicine.

The Role of Copper:
The copper ion (Cu²⁺) is not merely a passive passenger in this peptide. It is essential for GHK‑Cu’s biological activity. The copper ion acts as a critical cofactor, enabling the peptide to interact with cellular signaling pathways, bind to DNA sequences, and regulate gene expression. Without copper, the tripeptide GHK has significantly different properties.

GHK‑Cu 100 MG vs. 50 MG: Choosing the Right Quantity

Researchers often ask which quantity best suits their laboratory protocols. GHK‑Cu is available in both 50 mg and 100 mg vial sizes.

Choosing the Right Vial Size:

• GHK‑Cu 100 MG — Ideal for large‑scale experiments, high‑throughput screening, extended time‑course studies, and protocols requiring substantial peptide quantities with maximum cost efficiency. GHK-Cu 100MG

Both sizes share identical chemical properties, purity standards (≥99%), and quality assurance protocols.

Third‑Party Testing and Quality Assurance

Every batch of Premium GHK‑Cu 100 MG undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third‑party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release. GHK-Cu 100MG

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high‑performance liquid chromatography (HPLC) — ≥99%

• Copper content verification to confirm proper chelation

• Batch‑to‑batch consistency checks

• Endotoxin testing (<0.05 EU/mg) for cell culture applications

• Residual solvent analysis to confirm complete lyophilization

With a verified purity of ≥99%, GHK‑Cu meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. Batch‑specific Certificates of Analysis (CoA) are available upon request. GHK-Cu 100MG

Chemical and Physical Properties

Molecular Characteristics

GHK‑Cu is a copper‑binding tripeptide with a distinctive structure. The copper ion is chelated by the peptide, primarily through the histidine residue. GHK-Cu 100MG

Detailed Molecular Information:

• Molecular Formula: C₁₄H₂₄CuN₆O₄

• Molecular Mass: 401.93 g/mol

• CAS Number: 49557‑75‑7

• Sequence: Gly‑His‑Lys (glycine‑histidine‑lysine)

• Purity: ≥99% (HPLC)

• Appearance: Pale blue to blue‑green lyophilized powder

Amino Acid Properties:

Structural Features:

• Linear tripeptide (3 amino acids)

• Copper ion (Cu²⁺) chelated to the peptide

• Histidine residue provides primary coordination for copper

• Positively charged lysine residue

• Pale blue to blue‑green color due to copper content

Coordination Chemistry:
The copper ion is coordinated by the imidazole nitrogen of histidine, the amino terminus nitrogen, and two backbone amide nitrogens. This chelation creates a stable complex that protects the copper ion and enables specific biological interactions. GHK-Cu 100MG

Physical Form and Storage

The product is supplied as a lyophilized (freeze‑dried) powder within a sterile, sealed vial. The powder typically appears as a pale blue to blue‑green substance due to the copper content.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long‑term preservation (stable for ≥24 months)

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze‑thaw cycles once reconstituted

Stability Considerations:
GHK‑Cu is stable when stored properly in lyophilized form. The copper‑peptide bond is stable at neutral pH. Under acidic conditions (below pH 3.5), the copper may dissociate from the peptide. For most laboratory applications using neutral buffers (pH 7.0‑7.4), the complex remains intact.

Once reconstituted with an appropriate sterile solvent, the solution should be stored at 2‑8°C and used within 2‑4 weeks. For longer storage, freeze aliquots at -20°C or -80°C.

Intended Use and Regulatory Status

Research Use Only

GHK‑Cu is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating gene expression and cellular signaling

• Gene expression research — studying modulation of approximately 31% of human genes

• Extracellular matrix studies — collagen and elastin synthesis research

• Copper‑delivery studies — analyzing metal ion transport in culture

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into copper‑binding peptides

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations. GHK-Cu 100MG

Handling and Safety Guidelines

Professional Handling Required

GHK‑Cu should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15‑30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2‑5 minutes

Reconstitution Example for GHK‑Cu 100 MG:
For researchers needing a 100 mg/mL working solution, add 1 mL of sterile solvent to the 100 mg vial. For a 50 mg/mL solution, add 2 mL of solvent. For a 25 mg/mL solution, add 4 mL of solvent. For a 10 mg/mL solution, add 10 mL of solvent. Always verify calculations before proceeding with experiments. GHK-Cu 100MG

Solvent Considerations:
GHK‑Cu is water‑soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate‑buffered saline (PBS) at neutral pH (7.0‑7.4). Avoid acidic solvents (below pH 3.5) which may cause copper dissociation. GHK-Cu 100MG

Disposal Considerations

Dispose of GHK‑Cu and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines. GHK-Cu 100MG

Research Applications

GHK‑Cu is used in various laboratory research contexts, spanning genomics, cell biology, and tissue engineering. Typical applications include:

Gene Expression Studies:
GHK‑Cu is a well‑documented modulator of gene expression. Research has demonstrated that GHK‑Cu affects approximately 31% of human genes with ≥50% expression changes. This broad regulatory role makes GHK‑Cu valuable for studying:

• Gene expression patterns in response to peptide treatment

• Transcriptional regulation mechanisms

• Upregulation of beneficial genes (collagen, elastin, antioxidants)

• Downregulation of inflammatory genes

Collagen and Elastin Synthesis Research:
GHK‑Cu is known to stimulate the production of extracellular matrix components. Researchers use GHK‑Cu to study:

• Collagen type I synthesis and organization

• Elastin production and assembly

• Extracellular matrix remodeling dynamics

• Fibroblast activation and function

Wound Healing and Tissue Repair Studies:
GHK‑Cu has been extensively studied in tissue repair models. Research applications include:

• Wound healing kinetics and mechanisms

• Angiogenesis and blood vessel formation

• Granulation tissue formation

• Re‑epithelialization processes

Antioxidant and Anti‑Inflammatory Research:
GHK‑Cu modulates oxidative stress and inflammatory pathways. Researchers use GHK‑Cu to investigate:

• Antioxidant enzyme expression (SOD, catalase, glutathione peroxidase)

• Reactive oxygen species (ROS) regulation

• Pro‑inflammatory cytokine modulation

• NF‑κB pathway interactions

Fibroblast and Keratinocyte Cell Culture Studies:
GHK‑Cu is used in controlled cell culture systems to study:

• Fibroblast proliferation and migration

• Keratinocyte differentiation and function

• Cellular responses to copper‑peptide signaling

• Extracellular matrix production

Large‑Scale and High‑Throughput Applications:
The 100 MG vial size enables:

• High‑throughput screening assays requiring substantial peptide quantities

• Extended time‑course studies with consistent batch material

• Multi‑well plate experiments (384‑well, 1536‑well formats)

• Shared laboratory resources across multiple research teams

• Maximum cost efficiency for high‑volume research programs

Researchers should design their experimental protocols based on peer‑reviewed literature and established laboratory practices specific to copper‑binding peptides.

What Makes This Product Different

Naturally Occurring Human Peptide

Unlike many synthetic research peptides, GHK‑Cu is a naturally occurring peptide found in human plasma. This natural origin provides a relevant biological context for research into its mechanisms and functions. GHK-Cu 100MG

Broad Gene Expression Modulation

GHK‑Cu affects approximately 31% of human genes with ≥50% expression changes, making it one of the most broadly active regulatory peptides identified. This broad activity distinguishes GHK‑Cu from peptides with narrow, receptor‑specific actions.

Copper‑Dependent Activity

The copper ion is essential for GHK‑Cu’s biological activity. Unlike peptides that function solely through their amino acid sequence, GHK‑Cu requires proper copper chelation for gene regulatory and signaling functions.

High‑Capacity 100 MG Vials for Maximum‑Scale Research

The 100 MG vial size is specifically designed for researchers conducting large‑scale experiments, high‑throughput screening, extended multi‑week or multi‑month studies, and protocols requiring substantial peptide quantities. This highest capacity vial offers the maximum cost efficiency for extensive research programs. GHK-Cu 100MG

Rigorous Quality Control

Unlike research chemicals sold without verification, GHK‑Cu comes with documented third‑party testing for every batch, including HPLC purity analysis, mass spectrometry identity confirmation, and endotoxin testing (<0.05 EU/mg).

Distinctive Physical Appearance

The pale blue to blue‑green color of GHK‑Cu is a distinctive visual indicator of proper copper chelation, providing researchers with immediate visual confirmation of product integrity.

Batch Traceability

Each package of GHK‑Cu includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of GHK‑Cu 100 MG?

Each batch is tested to verify purity of ≥99% via HPLC, with identity confirmed by mass spectrometry. Individual certificates of analysis are available upon request.

What is the difference between GHK‑Cu 100 MG and 50 MG?

The only difference is the quantity per vial. GHK‑Cu 100 MG contains 100 mg per vial, while GHK‑Cu 50 MG contains 50 mg per vial. Both share identical chemical properties, purity standards (≥99%), and quality assurance protocols.

Which vial size should I choose for my research?

Choose 100 MG for large‑scale experiments, high‑throughput screening, extended time‑course studies, or protocols requiring higher peptide concentrations with maximum cost efficiency. Choose 50 MG for moderate‑scale studies and when precise handling of smaller quantities per vial is preferred.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Avoid repeated freeze‑thaw cycles once reconstituted. Lyophilized powder is stable for at least 24 months under recommended conditions.

What solvent should be used for reconstitution?

GHK‑Cu is water‑soluble. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral pH (7.0‑7.4). Avoid acidic solvents (pH < 3.5) which may cause copper dissociation. GHK-Cu 100MG

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. Endotoxin levels are confirmed at <0.05 EU/mg. For cell culture applications, additional filtration using a 0.22‑micron filter may be appropriate. GHK-Cu 100MG

What is the significance of the CAS number 49557‑75‑7?

The CAS number is a unique identifier for GHK‑Cu (Copper Tripeptide‑1), allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications. GHK-Cu 100MG

What is the amino acid sequence of GHK‑Cu?

The sequence is glycine‑histidine‑lysine, abbreviated as GHK or Gly‑His‑Lys.

Why does GHK‑Cu appear blue?

The pale blue to blue‑green color is characteristic of copper complexes. The copper ion (Cu²⁺) in GHK‑Cu absorbs light in the red‑orange region, resulting in the complementary blue color visible to researchers. GHK-Cu 100MG

What research applications is GHK‑Cu used for?

GHK‑Cu is studied in gene expression modulation, extracellular matrix remodeling, wound healing, angiogenesis, antioxidant pathways, and aging biology in laboratory cell culture and research models.

Can GHK‑Cu be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. GHK‑Cu is frequently studied in combination with BPC‑157 and TB‑500 in multi‑peptide formulations such as GLOW and KLOW. Standard laboratory practice includes testing interactions in control experiments before full‑scale implementation. GHK-Cu 100MG

Final Notes for Laboratory Researchers

Premium GHK‑Cu 100 MG represents a high‑purity research tool for qualified professionals conducting legitimate scientific investigations into gene expression modulation, extracellular matrix remodeling, tissue repair mechanisms, aging biology, and related regenerative fields. With documented third‑party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 100 mg vial size is specifically optimized for maximum‑scale research applications requiring substantial peptide quantities, including high‑throughput screening, extended time‑course studies, large‑scale multi‑well plate experiments, and protocols demanding consistent batch material across extensive research programs. As a naturally occurring human peptide with broad gene regulatory activity, GHK‑Cu offers unique research opportunities in regenerative biology and aging research.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training. GHK-Cu 100MG